[Recommendations for urinary organic acids analysis]

Area of Science:

• Biochemistry
• Clinical Chemistry
• Medical Diagnostics

Background:

• Hereditary metabolic diseases require comprehensive biochemical diagnosis.
• Metabolic profiling aids in detecting numerous compounds simultaneously.
• Organic acid chromatography is a key technique for identifying and quantifying these compounds.

Purpose of the Study:

• To propose a framework for accrediting organic acid chromatography.
• To align with medical biology accreditation standards (NF EN ISO 15189).
• To provide recommendations for validating this diagnostic method.

Main Methods:

• Discussing validation parameters specific to organic acid chromatography.
• Adhering to the recommendations of the French society for inborn errors of metabolism (SFEIM).
• Focusing on the accreditation process for medical biology examinations.

Main Results:

• The study outlines an approach for accrediting organic acid chromatography.
• It addresses the specific needs of diagnosing hereditary metabolic diseases.
• Recommendations cover essential validation parameters for reliable results.

Conclusions:

• Accreditation of organic acid chromatography is essential for accurate diagnosis of metabolic disorders.
• The proposed framework supports compliance with international standards.
• Standardized validation ensures the reliability of metabolic profiling in clinical settings.