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Updated: Dec 6, 2025

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure
Published on: June 25, 2010
[Recommendations for urinary organic acids analysis]
Laetitia Van Noolen1, Cécile Acquaviva-Bourdain2, Anne-Frédérique Dessein3
1Laboratoire des maladies héréditaires du métabolisme, Service de biochimie, biologie moléculaire et toxicologie environnementale, CHU Grenoble-Alpes, Grenoble, France.
Abstract:
Biochemical diagnosis of hereditary metabolic diseases requires the detection and simultaneous identification of a large number of compounds, hence the interest in metabolic profiles. Organic acid chromatography allows the identification of several hundred compounds and the quantification of the main molecules of interest. As part of the accreditation process for medical biology examinations according to standard NF EN ISO 15189, the group from the French society for inborn errors of metabolism (SFEIM) recommends an approach to accredit organic acid chromatography. Validation parameters and recommendations are discussed in this specific framework.
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