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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
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[Recommendations for aminoacids chromatography analysis].

Alice Veauville1, Cécile Acquaviva-Bourdain1, Marie Nowoczyn2

  • 1Service de biochimie et biologie moléculaire Grand Est - UM Pathologies métaboliques, érythrocytaires et dépistage périnatal, Centre de biologie Est, Hospices civils de Lyon, Bron, France.

Annales De Biologie Clinique
|October 7, 2020
PubMed
Summary

Accrediting amino acid chromatography is crucial for diagnosing hereditary metabolic diseases. This study outlines validation parameters and recommendations for this essential biochemical diagnostic method.

Keywords:
ISO 15189ISO standardaccreditationaminoacids chromatography

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Area of Science:

  • Biochemistry
  • Medical Diagnostics
  • Analytical Chemistry

Background:

  • Hereditary metabolic diseases require detecting numerous compounds for biochemical diagnosis.
  • Metabolic profiling and amino acid chromatography are key diagnostic tools.
  • Accreditation ensures the reliability of medical laboratory examinations.

Purpose of the Study:

  • To recommend an approach for accrediting amino acid chromatography.
  • To discuss validation parameters within the NF EN ISO 15189 framework.
  • To ensure accurate identification and quantification of amino acids.

Main Methods:

  • Amino acid chromatography for identifying and quantifying over forty compounds.
  • Validation parameter assessment according to NF EN ISO 15189 standards.
  • Methodological recommendations for accreditation.

Main Results:

  • A framework for accrediting amino acid chromatography has been proposed.
  • Key validation parameters and recommendations are detailed.
  • The study supports the reliable use of amino acid chromatography in diagnostics.

Conclusions:

  • Accreditation of amino acid chromatography is essential for accurate hereditary metabolic disease diagnosis.
  • The proposed approach ensures the quality and reliability of the method.
  • This work aids medical laboratories in meeting accreditation requirements.