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Protective Effect of Sodium Selenite on 4-Nonylphenol-Induced Hepatotoxicity and Nephrotoxicity in Rats

Murat Boyacioglu1, Ozay Gules2, Hande Sultan Sahiner3

  • 1Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, 09016, Isıklı, Aydın, Turkey. mboyacioglu@adu.edu.tr.

Biological Trace Element Research
|October 7, 2020
PubMed

Insights

Sodium selenite (SS) protects against 4-nonylphenol (4-NP) toxicity by improving DNA integrity and antioxidant status in rat liver and kidney tissues. SS supplementation mitigated 4-NP-induced damage, showing protective effects.

Area of Science:

  • Toxicology
  • Biochemistry
  • Histopathology

Background:

  • 4-nonylphenol (4-NP) is an environmental endocrine disruptor with known toxic effects.
  • Hepatotoxicity and nephrotoxicity are significant concerns associated with 4-NP exposure.
  • Antioxidant strategies are being explored to counteract xenobiotic-induced organ damage.

Purpose of the Study:

  • To evaluate the protective role of sodium selenite (SS) against 4-NP-induced toxicity.
  • To assess the impact of SS on DNA integrity, oxidative stress markers, and tissue histology in rats exposed to 4-NP.
  • To determine if SS can mitigate liver and kidney damage caused by 4-NP.

Main Methods:

  • Adult male Sprague Dawley rats were divided into four groups: control, SS, 4-NP, and SS+4-NP.
  • Treatments involved oral gavage administration of SS (0.5 mg/kg/day) and 4-NP (125 mg/kg/day) for 48 days.
  • Analyses included comet assay for DNA damage, oxidative stress parameter measurements, and histological examination of liver and kidney tissues.

Main Results:

  • SS administration significantly reduced DNA damage (% Tail DNA, Mean Tail Moment) in the SS+4-NP group compared to the 4-NP group.
  • SS treatment increased glutathione levels and decreased malondialdehyde levels in the SS+4-NP group.
  • Histological improvements were observed in the SS+4-NP group, including reduced liver cell degeneration and kidney glomerular damage.

Conclusions:

  • Sodium selenite demonstrates a significant protective effect against 4-nonylphenol-induced hepato-nephrotoxicity in rats.
  • SS mitigates 4-NP toxicity by preserving DNA integrity, enhancing antioxidant capacity, and improving tissue histology.
  • These findings suggest SS as a potential therapeutic agent against 4-NP-induced organ damage.
Keywords:
4-NonylphenolComet assayOxidative stressRatSodium seleniteToxicity

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