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Related Experiment Video

Updated: Dec 6, 2025

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Immunometabolism in the Single-Cell Era.

Maxim N Artyomov1, Jan Van den Bossche2

  • 1Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.

Cell Metabolism
|October 7, 2020
PubMed
Summary

Single-cell technologies reveal immune cell metabolism, uncovering how metabolic dysfunction drives disease. This advances understanding of immunometabolism for new diagnostics and therapeutics.

Keywords:
CyTOF mass cytometryflow cytometryimmunologyimmunometabolismmetabolismnext-generation immunometabolismsingle-cell RNA-sequencingspatiotemporal

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Area of Science:

  • Immunology
  • Metabolic research
  • Cellular biology

Background:

  • Metabolic pathways are vital for immune cell function and regulation.
  • Deranged metabolism in immune cells can lead to dysfunction and disease.
  • Previous insights were limited by bulk measurements, often using in vitro activated cells.

Purpose of the Study:

  • To review single-cell techniques for analyzing immune cell metabolism.
  • To highlight how these methods validate existing immunometabolism principles.
  • To identify context-dependent metabolic heterogeneity within immune cells.

Main Methods:

  • Review of emerging single-cell technologies.
  • Analysis of their application to clinical immune cell samples.
  • Comparison with traditional bulk measurement techniques.

Main Results:

  • Single-cell approaches enable metabolic state estimation in individual immune cells.
  • These techniques validate established immunometabolism concepts.
  • They reveal significant metabolic heterogeneity across different immune cell types and contexts.

Conclusions:

  • Single-cell analysis is revolutionizing immunometabolism research.
  • It offers a deeper understanding of immune cell metabolic states.
  • Future opportunities lie in developing novel therapeutic targets and diagnostic tools.