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The virtual circular genome model for primordial RNA replication.

Lijun Zhou1,2, Dian Ding1,3, Jack W Szostak1,2,3

  • 1Howard Hughes Medical Institute, Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

RNA (New York, N.Y.)
|October 8, 2020
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Summary
This summary is machine-generated.

This study proposes a model for primordial protocell genome replication using nonenzymatic RNA copying. It suggests genomes replicated via template-directed growth and rearrangements, potentially leading to the RNA World.

Keywords:
RNAnonenzymatic replicationoligonucleotidesorigin of lifeprotocell

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Area of Science:

  • Origin of Life Studies
  • Molecular Evolution
  • Biochemistry

Background:

  • Understanding early life requires models for primordial genome replication.
  • Nonenzymatic RNA copying provides a foundation for prebiotic replication mechanisms.

Purpose of the Study:

  • To propose a novel model for the replication of primordial protocell genomes.
  • To explore the potential for nonenzymatic RNA replication in early cellular systems.

Main Methods:

  • Modeling genome replication based on nonenzymatic RNA copying.
  • Considering template-directed oligonucleotide growth and in situ monomer activation.
  • Investigating the role of annealed configurations and spontaneous rearrangements.

Main Results:

  • Protocell genomes may have consisted of circular sequences with oligonucleotides at all positions.
  • Replication driven by monomer feeding and in situ activation.
  • Genome-wide replication possible with minimal average nucleotide addition.

Conclusions:

  • The proposed model offers a plausible mechanism for early genome replication.
  • This model supports the emergence of catalytically active ribozymes and the RNA World.
  • It provides a framework for understanding the transition from simple protocells to complex cells.