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Related Experiment Video

Updated: Dec 6, 2025

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

Published on: November 11, 2025

DNA-Barcoded Fluorescence Microscopy for Spatial Omics.

Florian Schueder^1,2, Eduard M Unterauer^1,2, Mahipal Ganji^1,2

¹Department of Physics and Center for Nanoscience, Ludwig Maximilian University, Geschwister-Scholl-Platz 1, Munich, 80539, Germany.

|October 8, 2020

Summary

This summary is machine-generated.

Spatial omics integrates molecular data with spatial context. DNA-barcoded fluorescence microscopy offers a promising approach for spatial genomics and transcriptomics, advancing single-cell analysis.

Keywords:

DNA nanotechnology fluorescence microscopy high-content imaging multiplexing super-resolution microscopy

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Last Updated: Dec 6, 2025

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Published on: November 11, 2025

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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Published on: July 6, 2022

Area of Science:

Life Sciences
Molecular Biology
Biotechnology

Background:

Genomics, transcriptomics, and proteomics generate vast quantitative biomolecule data.
Current methods struggle to link high-content molecular data with spatial information in cells and tissues.

Purpose of the Study:

Discuss current spatial omics implementations.
Review recent advancements in DNA-barcoded fluorescence microscopy.
Explore the potential of DNA-based imaging for spatial genomics, transcriptomics, and proteomics.

Main Methods:

Review of current spatial omics techniques.
Focus on DNA-barcoded fluorescence microscopy developments.
Analysis of high-resolution fluorescence microscopy for spatial proteomics.

Main Results:

Spatial omics techniques are advancing rapidly.
DNA-barcoded fluorescence microscopy shows significant potential for spatial genomics and transcriptomics.
Challenges remain in achieving high-resolution spatial proteomics.

Conclusions:

DNA-based imaging offers a powerful toolkit for spatial omics.
Further development is needed to overcome challenges in spatial proteomics.
High-resolution fluorescence microscopy is key to future spatial omics applications.