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Lipidomics and Transcriptomics in Neurological Diseases

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Cannabinoids Determination in Brain: A Supplemental Helpful in Postmortem Evaluation.

F Palazzoli¹, A L Santunione¹, F Marchesi¹

¹Department of Biomedical, Metabolic and Neural Sciences, Institute of Legal Medicine, University of Modena and Reggio Emilia, Modena, Italy.

Journal of Analytical Toxicology

|October 8, 2020

Summary

This summary is machine-generated.

This study developed a new LC-MS-MS method for analyzing cannabinoids like THC and CBD in postmortem blood and brain samples. The validated method provides reliable data to help determine the role of cannabis in deaths.

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On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

Published on: May 31, 2020

Area of Science:

Forensic Toxicology
Cannabinoid Analysis
Postmortem Investigations

Background:

Limited postmortem studies exist on cannabis and autopsy findings.
Existing research often analyzes few matrices or analytes.
Accurate quantification of cannabinoids in postmortem samples is crucial for forensic interpretation.

Purpose of the Study:

To develop and validate a sensitive LC-MS-MS method for simultaneous determination of key cannabinoids.
To analyze cannabinoids in central blood, femoral blood, and brain tissue from postmortem cases.
To provide reliable data for interpreting the role of cannabinoids in death investigations.

Main Methods:

Development of an optimized rapid clean-up procedure.
Utilized liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS).
Validated method for simultaneous determination of THC, CBD, 11-OH-THC, THC-COOH, and THC-COOH glucuronide.

Main Results:

Successfully developed and validated a sensitive and accurate LC-MS-MS method.
Applied the method to 24 postmortem cases involving cannabinoids.
Generated new data on cannabinoid concentrations, particularly in brain tissue.

Conclusions:

The validated LC-MS-MS method is reliable and accurate for postmortem cannabinoid analysis.
Combined analysis of blood and brain tissue provides a stronger basis for case interpretation.
Brain tissue analysis is a valuable supplement in determining the contribution of cannabinoids to mortality.