Achromobacter aestuarii sp. nov., Isolated from an Estuary

A novel strain KS-M25^T was isolated from estuary water in South Korea. Strain KS-M25^T was Gram-staining-negative, strictly aerobic, motile rods-shaped bacterium and showed oxidase- and catalase-positive reactions. Growth of strain KS-M25^T was observed at 10-25 °C (optimum, 20 °C), at pH 5.5-9.0 (optimum, pH 7.5), and with 0-6.0% (w/v) NaCl (optimum, 1%). Ubiquinone-8 was identified as the sole isoprenoid quinone and the major fatty acids were C_16:0, cyclo-C_17:0 and sum in feature 3 (comprising C_16:1 ω7c and/or C_16:1 ω6c). The G+C content values based on genome sequences was 62.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KS-M25^T formed a clear cluster within the genus Achromobacter. Strain KS-M25^T was most closely related to Achromobacter anxifer LMG 26857^T, Achromobacter dolens LMG 26840^T, and Achromobacter xylosoxidans NBRC 15126^T with 97.8%, 97.8%, and 97.7% 16S rRNA gene sequence similarities, respectively. Based on phenotypic, chemotaxonomic, and phylogenetic analysis, strain KS-M25^T represents a novel species of the genes Achromobacter, for which the name Achromobacter aestuarii sp. nov. is proposed. The type strain is KS-M25^T (= KACC 21219^T = JCM 33329^T).