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Effect of different human tissue processing techniques on SARS-CoV-2 inactivation-review
André Oliveira Paggiaro1,2,3, Viviane Fernandes Carvalho4, Rolf Gemperli5
1ICHC Tissue Bank-HCFMUSP, Eneas de Carvalho, São Paulo, 05403-000, Brazil. andre.paggiaro@hc.fm.usp.br.
Cell and Tissue Banking
|October 9, 2020
Summary
Tissue banks must screen donors rigorously due to SARS-CoV-2 infectivity in tissues like skin and cornea. Gamma irradiation shows promise for sterilizing certain tissues against the virus.
Area of Science:
- Virology
- Tissue Engineering
- Public Health
Background:
- The emergence of SARS-CoV-2 poses risks for tissue transplant safety.
- Understanding viral infectivity in biological tissues is crucial.
Purpose of the Study:
- To review SARS-CoV-2 infectivity in various tissues.
- To assess the impact of processing on viral inactivation.
Main Methods:
- Literature review on SARS-CoV-2 and tissue processing.
- Analysis of viral binding to ACE2 receptors in human tissues.
Main Results:
- SARS-CoV-2 infects tissues expressing ACE2 receptors (skin, musculoskeletal, amniotic, cardiovascular, ocular).
- Coronaviruses are stable at low temperatures; inactivated by heat and pH.
- Gamma irradiation effective for SARS/MERS inactivation, potentially useful for SARS-CoV-2.
Conclusions:
- Limited data exists on tissue processing's effect on viral neutralization.
- Rigorous donor screening remains essential for recipient safety.
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