Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Determination

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The ALX1 transcription factor acts in the early cranial mesoderm to specify extraocular muscle formation.

Disease models & mechanisms·2026

Same author

Twist1 Acts Upstream of the Dlx5-Hand2 Pathway to Pattern the Mammalian Jaw.

Journal of dental research·2024

Same author

Lineage-specific requirements of Alx4 function in craniofacial and hair development.

Developmental dynamics : an official publication of the American Association of Anatomists·2024

Same author

Reduced Nephron Endowment in Six2-TGCtg Mice Is Due to Six3 Misexpression by Aberrant Enhancer-Promoter Interactions in the Transgene.

Journal of the American Society of Nephrology : JASN·2024

Same author

The transcription factors Foxf1 and Foxf2 integrate the SHH, HGF and TGFβ signaling pathways to drive tongue organogenesis.

Development (Cambridge, England)·2022

Same author

MSX1 Drives Tooth Morphogenesis Through Controlling Wnt Signaling Activity.

Journal of dental research·2022

Search research articles

Related Experiment Video

Updated: Dec 6, 2025

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Published on: February 13, 2021

Tissue-specific analysis of Fgf18 gene function in palate development.

Minghui Yue¹, Yu Lan^1,2,3,4,5, Han Liu¹

¹Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|October 9, 2020

Summary

Fibroblast growth factor 18 (Fgf18) is crucial for mandible and craniofacial bone development in neural crest cells. However, Fgf18 is not essential for palatogenesis, despite its role in cleft palate defects.

Keywords:

bone cleft palate conditional gene knockout mouse neural crest

More Related Videos

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Live Imaging of Mouse Secondary Palate Fusion

Live Imaging of Mouse Secondary Palate Fusion

Published on: July 27, 2017

Related Experiment Videos

Last Updated: Dec 6, 2025

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes

Published on: February 13, 2021

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Live Imaging of Mouse Secondary Palate Fusion

Live Imaging of Mouse Secondary Palate Fusion

Published on: July 27, 2017

Area of Science:

Developmental biology
Craniofacial development
Molecular biology

Background:

Previous studies linked Fgf18 to cleft palate in mice and humans.
The precise function of Fgf18 during palatogenesis remained unclear.

Purpose of the Study:

To investigate the specific roles of Fgf18 during mouse palatogenesis.
To determine the necessity of Fgf18 in neural crest-derived mesenchyme for craniofacial development.

Main Methods:

Utilized tissue-specific inactivation of Fgf18 in neural crest-derived craniofacial mesenchyme using Fgf18^c/c ;Wnt1-Cre mice.
Analyzed craniofacial skeletal elements, palatal shelf elevation, and cell proliferation.
Performed embryonic maxillary explant cultures.

Main Results:

Fgf18 inactivation in neural crest mesenchyme led to shortened mandibles and reduced ossification of cranial bones.
Approximately 64% of mutant mice exhibited cleft palate, with impaired palatal shelf elevation.
Palatal cell proliferation and shelf fusion in organ culture were not significantly affected by Fgf18 inactivation in palatal mesenchyme.

Conclusions:

Fgf18 expression in neural crest-derived mesenchyme is critical for mandible and craniofacial bone development.
Fgf18 is dispensable for palatogenesis, despite its association with cleft palate defects.