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Updated: Dec 6, 2025

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
Mechanistic studies on the role of TGF-β1 in angiogenesis through EndMT
Sheng-Jun Cao1, Lei Hong2, Xiao-Qiang Li3
1Department of Vascular Surgery, Taizhou Second People's Hospital, Jiangsu, China.
Objective:
This study aims to investigate the mechanism of transforming growth factor-β1 (TGF-β1) in promoting angiogenesis through endothelial-to-mesenchymal transition (EndMT).
Methods:
The mesenchymal transition of human umbilical vein endothelial cells (HUVECs) was induced by TGF-β1. The angiogenesis, migration, and proliferation of HUVECs undergoing EndMT were examined by tube formation assay, scratch assay, Transwell assay, and CCK-8 assay.
Results:
The outcomes revealed that EndMT promoted angiogenesis, migration, and proliferation of HUVECs and the secretion of the vascular endothelial growth factor (VEGF) of HUVECs. Phosphorylated AKT (p-AKT) increased in EndMT by inhibiting the mitigation of angiogenesis.
Conclusion:
EndMT induces angiogenesis by promoting the secretion of VEGF, and p-AKT participates in this regulation.
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