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Related Concept Videos

Ribozymes02:47

Ribozymes

13.1K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
13.1K
Ribozymes02:47

Ribozymes

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3.2K
Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Weak effects of prebiotically plausible peptides on self-triphosphorylation ribozyme function.

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Related Experiment Video

Updated: Dec 6, 2025

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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A combinatorial method to isolate short ribozymes from complex ribozyme libraries.

Joshua T Arriola1, Ulrich F Müller1

  • 1Department of Chemistry & Biochemistry, University of California San Diego, La Jolla, California 92093, USA.

Nucleic Acids Research
|October 9, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to find short catalytic RNAs (ribozymes). This technique identified a 17-nucleotide ribozyme motif, useful for biochemical tools and understanding early life.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Origin of Life Studies

Background:

  • In vitro selection is key to discovering novel catalytic RNAs (ribozymes) not found in nature.
  • Short ribozymes are valuable as biochemical tools and for insights into prebiotic chemistry.
  • Previous work identified ribozymes catalyzing triphosphorylation using cyclic trimetaphosphate, with the shortest being 67 nucleotides.

Purpose of the Study:

  • To develop a combinatorial method for identifying shorter ribozymes from large libraries.
  • To apply this method to find minimal functional ribozyme sequences.

Main Methods:

  • A combinatorial approach was employed to screen a library of triphosphorylation ribozymes.
  • The method focuses on identifying short, functional sequence motifs within larger structures.

Main Results:

  • A 17-nucleotide sequence motif was identified within a 44-nucleotide pseudoknot structure.
  • This represents a significant reduction in length compared to previously identified ribozymes from the same library.

Conclusions:

  • The developed combinatorial method is effective for discovering short ribozymes.
  • This approach can be applied to analyze diverse libraries from in vitro selection experiments.
  • The findings contribute to the development of RNA-based tools and understanding RNA's role in early life.