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Related Concept Videos

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The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
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Related Experiment Video

Updated: Dec 6, 2025

Analysis of Somatic Hypermutation in the JH4 intron of Germinal Center B cells from Mouse Peyer's Patches
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PHF14 is required for germinal center B cell development.

Le Zhang1, Yanlai Lu2, Yuliang Wang2

  • 1Department of Immunology, Key Laboratory of Immune Microenvironment and Diseases, Nanjing Medical University, Nanjin 211166, China; Analysis Center, Nanjing Medical University, Nanjing 211166, China.

Cellular Immunology
|October 9, 2020
PubMed
Summary

PHF14 is crucial for germinal center (GC) formation in spleen immunity. This study shows PHF14 regulates GC B cell proliferation, impacting humoral immunity and antibody diversification.

Keywords:
Germinal centerPHF14Proliferationp21

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Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Germinal centers (GCs) are essential for humoral immunity, antibody diversification, and affinity maturation.
  • GC B cell proliferation is critical for somatic hypermutation and positive selection processes.

Purpose of the Study:

  • To investigate the role of PHF14 in GC formation and B cell proliferation.
  • To understand the molecular mechanisms by which PHF14 regulates GC B cells.

Main Methods:

  • Generation of PHF14-deficient mice (PHF14GCB KO) specifically in GC B cells.
  • Analysis of GC response in spleens after SRBC or lymphocytic choriomeningitis virus challenge.
  • Assessment of B cell survival, proliferation, and gene expression (Cdkn1a, H3K4me3).

Main Results:

  • PHF14 deficiency significantly reduced GC response in mouse spleens.
  • PHF14 regulates GC B cell proliferation but not cell survival.
  • PHF14 suppresses Cdkn1a (p21) expression by modulating H3K4me3 levels, thereby controlling GC B cell proliferation.

Conclusions:

  • PHF14 plays a significant role in germinal center formation.
  • PHF14 regulates GC B cell proliferation through epigenetic mechanisms involving H3K4me3 and Cdkn1a.
  • PHF14 is a key regulator of humoral immune responses.