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Updated: Dec 6, 2025
Using Single-Worm Data to Quantify Heterogeneity in Caenorhabditis elegans-Bacterial Interactions
Published on: July 22, 2022
Reduced gastrointestinal worm burden following long term parasite control improves body condition and fertility in
Jerimyah Johnson1, Vanmathy R Kasimanickam1, John P Kastelic2
1Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA.
Abstract:
The objective of this study was to compare effects of gastrointestinal parasite control over a long vs short term (PC-LT vs. PC-ST) on fecal parasite load, body condition and pregnancy in beef cows. On Day 0, fecal samples were collected from Angus cross cows (n = 1462) and they were assigned a body condition score (BCS: 1, emaciated; 9, Obese) and randomly divided into two groups (within location) to receive extended-release eprinomectin [PC-LT; n = 749; 50 mg/50 kg body weight (BW)] or pour-on ivermectin (PC-ST; n = 713; 25 mg/50 kg BW). All cows were synchronized with CO-Synch + CIDR [100 μg GnRH + progesterone vaginal insert (CIDR) application on Day 20, CIDR removal +25 mg PGF2a on Day 27, and artificial insemination +100 μg GnRH on Day 30 (66 h after CIDR removal)] protocol, artificially inseminated (AI; Day 30) and on Day 44, exposed to breeding bulls (1:40 bull to cow ratio) for the remainder of the 85 day breeding season. On Day 90, a second fecal sample was collected from all cows and the cows were examined to determine pregnancy/AI (P/AI). All cows were assigned a BCS on Day 180 and re-examined to determine pregnancy/breeding season (P/BS). Worm egg count per gram of feces (FEG) was determined by McMaster method. There were no differences (P > 0.1) between PC-LT and PC-ST groups on Day 0 for FEG (46.9 ± 13.1 vs 42.6 ± 15.2, respectively; mean ± SEM) or BCS (5.95 ± 0.12 vs 6.00 ± 0.20). The mean FEG (PC-LT, 12.3 ± 4.7 vs. PC-ST, 131.3 ± 10.9) on Day 90 and BCS (PC-LT, 6.04 ± 0.07 vs. PC-ST, 5.79 ± 0.13) on Day 180 differed (P < 0.05) between the two groups. Mean P/AI [PC-LT, 62.9 %; (471/749) vs PC-ST, 57.4 %; (409/713)] and P/BS [PC-LT, 92.9 % (696/749) vs PC-ST, 90.0 (642/713)] also differed (P < 0.05). Lower FEG at Day 90 resulted in moderate to good body condition at Day 180 and cows with moderate to good body condition at Day 180 had higher P/BS. In conclusion, lower worm burden with long-term parasite control reduced FEG and improved BCS, P/AI and P/BS.
