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Profiling of specific long non-coding RNA signatures identifies ST8SIA6-AS1 AS a novel target for breast cancer

Zhanghan Chen1, Jie Huang1, Yanling Feng2

  • 1Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.

The Journal of Gene Medicine
|October 10, 2020
PubMed
Abstract

Insights

Long non-coding RNA ST8SIA6-AS1 promotes breast cancer progression by enhancing cell proliferation, migration, and invasion. This study identifies ST8SIA6-AS1 as a potential therapeutic target for breast cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer remains a leading cause of cancer death in women, with unmet needs in diagnosis and treatment.
  • Long non-coding RNAs (lncRNAs) are implicated in tumor development, presenting potential therapeutic targets.
  • This study investigates lncRNA expression profiles to identify novel therapeutic targets for breast cancer.

Purpose of the Study:

  • To identify differentially expressed lncRNAs in breast cancer.
  • To investigate the functional role of the lncRNA ST8SIA6-AS1 in breast cancer.
  • To explore potential molecular mechanisms of ST8SIA6-AS1 in breast cancer progression.

Main Methods:

  • Competitive endogenous RNA (ceRNA) microarray analysis to profile lncRNAs.
  • Quantitative real-time polymerase chain reaction (qRT-PCR) to validate differentially expressed lncRNAs.
  • Cell function assays and bioinformatics analyses to elucidate ST8SIA6-AS1 function and interactions.

Main Results:

  • Seven lncRNAs, including ST8SIA6-AS1, were significantly upregulated in breast cancer tissues.
  • ST8SIA6-AS1 expression correlated with TNM staging and Ki-67 index.
  • ST8SIA6-AS1 promoted breast cancer cell proliferation, migration, and invasion in vitro.

Conclusions:

  • lncRNA ST8SIA6-AS1 promotes breast cancer cell proliferation, migration, and invasion.
  • ST8SIA6-AS1 may contribute to breast cancer progression via the miR-4252 pathway or interaction with RNA binding proteins (NONO, QKI, RBMX).
  • ST8SIA6-AS1 represents a potential therapeutic target for breast cancer.
Keywords:
ST8SIA6-AS1biomarkerbreast cancerlong non-coding RNA

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