In vitro effects of CaO nanoparticles on Triticale callus exposed to short and long-term salt stress

Ca²⁺ NPs enhanced tolerance of Triticale callus under salt stress by improving biochemical activity and confocal laser scanning analysis, conferring salt tolerance on callus cells. CaO NPs (Ca²⁺) are significant components that act as transducers in many adaptive and developmental processes in plants. In this study, effect of Ca²⁺ NPs on the response and regulation of the protective system in Triticale callus under short and long-salt treatments was investigated. The activation of Ca²⁺ NPs was induced by salt stress in callus of Triticale cultivars. MDA, H₂O₂, POD, and protein activities were determined in callus tissues. Concerning MDA, H₂O₂, protein activities, it was found that the Ca²⁺ NPs treatment was significant, and it demonstrated a high correlation with the tolerance levels of cultivars. Tatlıcak cultivar was detected for better MDA activities in the short time with 1.5 ppm Ca²⁺ NPs concentration of 50 g and 100 g NaCl. Similarly, the same cultivar responded with better H₂O₂ activity at 1.5 ppm Ca²⁺ NPs 100 g NaCl in the short time. POD activities exhibited a decreasing trend in response to the increasing concentrations of Ca²⁺ NPs. The best result was observed at 1.5 ppm Ca²⁺ NPs 100 g NaCl in the short term. Based on the protein content, treatment of short-term cultured callus cells with 1.5 ppm Ca²⁺ NPs inhibited stress response and it significantly promoted Ca²⁺ NPs signals as compared to control callus. Confocal laser scanning analysis proved that the application of Ca²⁺ NPs could alleviate the adverse effects of salt stress by the inhibition of stress severity in callus cells. This study demonstrated, under in vitro conditions, that the application of Ca²⁺ NPs can significantly suppress the adverse effects of salt stress on Triticale callus; it was also verified that the concentration of Ca²⁺ NPs could be important parameter to be considered in adjusting the micronutrient content in the media for this plant.