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Circ_0000285 regulates proliferation, migration, invasion and apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis

Zhisheng Long1, Feipeng Gong1, Yuxu Li1

  • 1Department of Orthopaedics, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, No.92, AiGuo Road, Nanchang City, 330006 Jiangxi Province China.

Cancer Cell International
|October 12, 2020
PubMed
Abstract

Insights

Circular RNAs (circRNAs) like circ_0000285 are upregulated in osteosarcoma (OS). Circ_0000285 promotes OS progression by sponging miR-409-3p, which targets IGFBP3, indicating circ_0000285 is a potential therapeutic target for OS.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are critical in disease pathogenesis.
  • The specific role of circRNAs in osteosarcoma (OS) remains largely unknown.
  • Investigating circRNAs offers potential for novel therapeutic strategies in OS.

Purpose of the Study:

  • To elucidate the function of circ_0000285 in osteosarcoma (OS).
  • To investigate the molecular mechanism of circ_0000285 in OS progression.
  • To explore the potential of circ_0000285 as a therapeutic target for OS.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and western blot to assess expression levels.
  • Cell proliferation, apoptosis, invasion, and migration assays (CCK-8, colony formation, flow cytometry, Transwell).
  • Dual-luciferase reporter and RNA immunoprecipitation (RIP) assays to confirm molecular interactions; in vivo animal experiments.

Main Results:

  • Circ_0000285 expression is significantly elevated in OS tissues and cells.
  • Knockdown of circ_0000285 suppresses OS cell proliferation, migration, and invasion, while promoting apoptosis, both in vitro and in vivo.
  • Circ_0000285 acts as a sponge for miR-409-3p, which in turn targets IGFBP3, forming the circ_0000285/miR-409-3p/IGFBP3 axis that drives OS progression.

Conclusions:

  • Circ_0000285 significantly influences osteosarcoma cell proliferation, migration, invasion, and apoptosis via the miR-409-3p/IGFBP3 pathway.
  • Circ_0000285 presents a promising molecular target for the development of novel therapeutic interventions for osteosarcoma.
Keywords:
Cell growthIGFBP3OScirc_0000285miR-409-3p

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