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Related Experiment Video

Updated: Dec 6, 2025

In Vivo Two-photon Imaging of Megakaryocytes and Proplatelets in the Mouse Skull Bone Marrow
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Quantitative Optical Diffraction Tomography Imaging of Mouse Platelets.

Tess A Stanly1, Rakesh Suman2, Gulab Fatima Rani3

  • 1York Biomedical Research Institute, Department of Biology, University of York, York, United Kingdom.

Frontiers in Physiology
|October 12, 2020
PubMed
Summary
This summary is machine-generated.

Holotomography enables analysis of individual live platelets, revealing morphological and biophysical differences in disease states like myeloproliferative neoplasms and parasitic infections. This advanced imaging technique offers new insights into platelet function and activation.

Keywords:
JAK2V617FMPNholotomographyleishmaniasisplatelets

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Area of Science:

  • Hematology
  • Biophysics
  • Immunology

Background:

  • Platelets are crucial for hemostasis and increasingly recognized for roles in immunity and inflammation.
  • Pathological conditions like parasitic infections and myeloid malignancies involve platelets as mediators of hemostatic and inflammatory responses.
  • Studying murine platelet function is vital, but traditional methods using whole populations limit assay types and sample analysis.

Purpose of the Study:

  • To introduce and validate 3D quantitative phase imaging (holotomography) for analyzing single, unlabeled, live mouse platelets.
  • To investigate morphological and biophysical differences in platelets from mice with JAK2V617F-positive myeloproliferative neoplasm (MPN) or Leishmania donovani infection compared to controls.
  • To assess the utility of holotomography in identifying disparities in platelet activation status and functional ability in disease states.

Main Methods:

  • Utilized optical diffraction tomography (ODT) for 3D quantitative phase imaging (holotomography) of single, live platelets.
  • Analyzed platelets isolated from whole blood of mice with JAK2V617F-MPN, Leishmania donovani infection, and healthy controls.
  • Quantified morphological parameters (volume, sphericity) and biophysical properties (refractive index, dry mass) of individual platelets.

Main Results:

  • Holotomography successfully identified and quantified differences in single, unlabeled, live platelets with minimal interference.
  • Significant alterations in platelet morphology, including cell volume and sphericity, were observed in disease states.
  • Biophysical parameters such as refractive index and dry mass showed distinct changes, indicating altered platelet states in MPN and parasitic infection.

Conclusions:

  • Holotomography is a powerful, non-invasive technique for analyzing single platelets, offering detailed morphological and biophysical insights.
  • This method reveals previously uncharacterized differences in platelet characteristics associated with specific disease conditions.
  • Holotomography facilitates the identification of disparities in platelet activation status and potential functional capacity at the single-cell level in disease models.