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Transcriptomic Analysis of Two Lentinula edodes Genotypes With Different Cadmium Accumulation Ability.

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Area of Science:

  • Mycology
  • Environmental Science
  • Molecular Biology

Background:

  • Lentinula edodes (Shiitake mushroom) is a popular edible mushroom worldwide.
  • L. edodes is susceptible to accumulating toxic heavy metals, particularly cadmium (Cd).
  • Understanding Cd accumulation mechanisms is crucial for food safety and potential bioremediation applications.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying differential cadmium (Cd) accumulation in two Lentinula edodes genotypes.
  • To identify key genes and pathways involved in Cd enrichment in L. edodes.
  • To provide insights for developing L. edodes strains with reduced Cd uptake.

Main Methods:

  • Comparative transcriptomic analysis using RNA-Seq on two L. edodes genotypes (Le4625 with high Cd accumulation and Le4606 with low Cd accumulation) exposed to Cd.
  • Analysis of differentially expressed genes (DEGs) in response to Cd exposure and between the two genotypes.
  • Identification of candidate genes associated with Cd accumulation.

Main Results:

  • Significant differences in Cd concentrations were observed between Le4625 and Le4606 mycelia after Cd exposure, with Le4625 accumulating approximately three-fold more Cd.
  • Cd exposure induced differential gene expression in both genotypes, with Ld4606 showing more Cd-induced transcriptional changes.
  • DEGs between genotypes after Cd exposure were linked to transmembrane transport, oxidative stress response, signaling pathways, and cell wall modification.
  • Candidate genes, including major facilitator superfamily genes, heat shock proteins, and laccase 11, were identified as potentially involved in Cd accumulation.

Conclusions:

  • Cadmium enrichment in L. edodes involves complex molecular mechanisms including MAPK signaling and anti-oxidative stress responses.
  • The study identified candidate genes that regulate Cd accumulation in L. edodes.
  • Findings support the development of L. edodes strains with lower Cd enrichment for safer consumption and for engineered applications in environmental remediation.