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Improvements of Western blotting to detect monoclonal antibodies

M Bestagno, A Cerino, S Riva

    Biochemical and Biophysical Research Communications
    |August 14, 1987
    PubMed

    A comparison of the effects of different factors on the sensitivity of Western blotting technique to detect monoclonal antibodies is described. The major improvements were obtained by: A) renaturating the antigen in the gel before transferring it in carbonate buffer at pH 10 onto nitrocellulose and B) using alkaline-phosphatase-conjugated second antibody instead of peroxidase-conjugated second antibody.

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