Patient-Derived Tumor Organoid Rings for Histologic Characterization and High-Throughput Screening
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Updated: Dec 6, 2025
Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
Published on: December 23, 2022
Huyen Thi Lam Nguyen1,2, Alice Soragni1,3,4,5,6
1Department of Orthopaedic Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Abstract:
Tumor organoids are promising tools for cancer biology investigations and preclinical drug screenings because they are often representative of the histology and drug responses of patients. Here, we introduce a facile protocol to overcome technical limitations by generating patient-derived tumor organoids using a simplified ring-like geometry. This facilitates media exchange and drug treatment for histopathology characterization and automated high-throughput drug screenings. For complete details on the use and execution of this protocol, please refer to Phan et al. (2019).
Insights
Patient-derived tumor organoids offer insights into cancer biology and drug responses. A new simplified ring-like geometry protocol enhances their utility for high-throughput drug screening and histopathology.
Area of Science:
- Biomedical Engineering
- Cancer Research
- Drug Discovery
Background:
- Patient-derived tumor organoids are valuable models for cancer research due to their ability to recapitulate patient-specific histology and drug sensitivity.
- Existing methods for generating and utilizing tumor organoids face technical limitations that hinder their application in high-throughput screening.
- There is a need for improved protocols to enhance the efficiency and effectiveness of tumor organoid models.
Purpose of the Study:
- To introduce a facile protocol for generating patient-derived tumor organoids with a simplified ring-like geometry.
- To overcome technical limitations associated with traditional tumor organoid culture and drug screening.
- To facilitate automated high-throughput drug screening and histopathology characterization of tumor organoids.
Main Methods:
- Development of a novel, simplified ring-like geometry for culturing patient-derived tumor organoids.
- Implementation of the protocol to generate tumor organoids from patient samples.
- Adaptation of the organoid culture system for efficient media exchange and drug treatment.
- Integration of the system for automated high-throughput drug screening and histopathological analysis.
Main Results:
- Successful generation of patient-derived tumor organoids using the simplified ring-like geometry.
- Demonstrated enhanced efficiency in media exchange and drug delivery compared to conventional methods.
- Facilitation of automated high-throughput drug screening assays.
- Enabled detailed histopathological characterization of the generated tumor organoids.
Conclusions:
- The novel ring-like geometry protocol provides a facile and effective method for generating patient-derived tumor organoids.
- This approach overcomes key technical limitations, improving the utility of tumor organoids for preclinical drug screening and cancer research.
- The protocol supports automated high-throughput applications, accelerating the discovery of novel cancer therapies.
