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Updated: Dec 6, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing
Weimin Tian1, Xiaoling Liu, Gaoting Wang
1Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, Shaanxi Province, P. R. China. liuxl2018@snnu.edu.cn liuch@snnu.edu.cn.
Abstract:
A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies.
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