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Updated: Dec 6, 2025

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors
Published on: January 31, 2025
Cytoskeleton systems contribute differently to the functional intrinsic properties of chondrospheres
Anna A Gryadunova1, Elizaveta V Koudan2, Sergey A Rodionov3
1Laboratory for Biotechnological Research 3D Bioprinting Solutions, Moscow 115409, Russian Federation; Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russian Federation.
Abstract:
Cytoskeleton systems, actin microfilaments, microtubules (MTs) and intermediate filaments (IFs) provide the biomechanical stability and spatial organization in cells. To understand the specific contributions of each cytoskeleton systems to intrinsic properties of spheroids, we've scrutinized the effects of the cytoskeleton perturbants, cytochalasin D (Cyto D), nocodazole (Noc) and withaferin A (WFA) on fusion, spreading on adhesive surface, morphology and biomechanics of chondrospheres (CSs). We confirmed that treatment with Cyto D but not with Noc or WFA severely affected CSs fusion and spreading dynamics and significantly reduced biomechanical properties of cell aggregates. Noc treatment affected spheroids spreading but not the fusion and surprisingly enhanced their stiffness. Vimentin intermediate filaments (VIFs) reorganization affected CSs spreading only. The analysis of all three cytoskeleton systems contribution to spheroids intrinsic properties was performed for the first time.
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