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Updated: Dec 6, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Optimization of nitrogen and carbon removal with simultaneous partial nitrification, anammox and denitrification in
Kai Zhang1, Zhaozhao Wang2, Mengxia Sun1
1National Engineering Laboratory of Urban Sewage Advanced Treatment and Resource Utilization Technology, The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, People's Republic of China.
Abstract:
In this study, a membrane bioreactor (MBR) was used to achieve both nitrogen and carbon removal by a simultaneous partial nitrification, anammox and denitrification (SNAD) process. During the entire experiment, the intermittent aeration (non-aerobic time : aeration time, min min-1) cycle was controlled by a time-controlled switch, and the aeration rate was controlled by a gas flowmeter, and the optimal operating parameters as determined by response surface methodology (RSM) were a C/N value of 1.16, a DO value of 0.84 mg l-1 and an aerobic time (T ae) of 15.75 min. Under these conditions, the SNAD process achieved efficient and stable nitrogen and carbon removal; the total inorganic nitrogen removal efficiency and chemical oxygen demand removal efficiency were 92.31% and 95.67%, respectively. With the formation of granular sludge, the membrane fouling rate decreased significantly from 35.0 Pa h-1 at SNAD start-up to 19.9 Pa h-1 during stable operation. Fluorescence in situ hybrid analyses confirmed the structural characteristics and the relative ratio of aerobic ammonia-oxidizing bacteria, anaerobic ammonia-oxidizing bacteria and denitrifying bacteria in the SNAD system.
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