Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.4K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.4K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.0K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.0K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.0K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.0K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.6K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.6K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

10.0K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface Modified β-Ti-18Mo-6Nb-5Ta (wt%) Alloy for Bone Implant Applications: Composite Characterization and Cytocompatibility Assessment.

Journal of functional biomaterials·2023
Same author

Biocompatibility and Electrical Stimulation of Skeletal and Smooth Muscle Cells Cultured on Piezoelectric Nanogenerators.

International journal of molecular sciences·2022
Same author

Transgenic mice overexpressing the PGE<sub>2</sub> receptor EP<sub>2</sub> on mast cells exhibit a protective phenotype in a model of allergic asthma.

Allergy·2021
Same author

ZnO Nanosheet-Coated TiZrPdSiNb Alloy as a Piezoelectric Hybrid Material for Self-Stimulating Orthopedic Implants.

Biomedicines·2021
Same author

Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells.

International journal of molecular sciences·2021
Same author

Endogenous CRISPR/Cas9 arrays for scalable whole-organism lineage tracing.

Development (Cambridge, England)·2020

Related Experiment Video

Updated: Dec 6, 2025

Generation of Mice Derived from Induced Pluripotent Stem Cells
11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

22.1K

Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features.

Marta Vila-Cejudo1,2, Sandra Alonso-Alonso1, Anna Pujol3

  • 1Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.

Journal of Assisted Reproduction and Genetics
|October 13, 2020
PubMed
Summary

Modulating Wnt and MAPK pathways is crucial for deriving mouse embryonic stem cells (mESCs) from single blastomeres. Different pathway combinations yield mESCs with varying pluripotency features, impacting their developmental potential.

Keywords:
MAPK signaling pathwayMouse embryonic stem cellsPluripotency stateSingle blastomeresWnt signaling pathway

More Related Videos

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency
08:08

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Published on: April 22, 2011

10.8K
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.7K

Related Experiment Videos

Last Updated: Dec 6, 2025

Generation of Mice Derived from Induced Pluripotent Stem Cells
11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

22.1K
Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency
08:08

Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Published on: April 22, 2011

10.8K
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.7K

Area of Science:

  • Stem cell biology
  • Developmental biology
  • Molecular signaling pathways

Background:

  • Mouse embryonic stem cells (mESCs) are critical for developmental studies.
  • Deriving mESCs from single blastomeres presents unique challenges.
  • Understanding signaling pathways involved in pluripotency is essential.

Purpose of the Study:

  • To investigate the role of the Wnt pathway in mESC derivation from single blastomeres.
  • To assess the impact of Wnt and MAPK pathway modulation on mESC pluripotency.
  • To determine the features of mESCs derived under different signaling conditions.

Main Methods:

  • Utilized Wnt activator CHIR99021 and Wnt inhibitor IWR-1-endo.
  • Employed MEK inhibitor PD0325901 for pathway modulation.
  • Assessed alkaline phosphatase activity, FGF5 levels, gene expression, and chimera formation.

Main Results:

  • Combined Wnt and MEK inhibition strategies significantly increased derivation efficiency.
  • Full naïve pluripotency was achieved with specific Wnt/MAPK pathway activations.
  • Partial pathway modulation resulted in mixed naïve-like and primed-like pluripotency features.

Conclusions:

  • Wnt and MAPK pathways are pivotal for deriving mESCs from single blastomeres.
  • Targeted modulation of these pathways generates mESC lines with distinct pluripotency characteristics.
  • This study provides insights into controlling mESC derivation and pluripotency states.