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CRISPR/Cas-based precision genome editing via microhomology-mediated end joining
Tien Van Vu1,2, Duong Thi Hai Doan1, Jihae Kim1
1Division of Applied Life Science (BK21 Plus Program), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Plant Biotechnology Journal
|October 13, 2020
Summary
Precision gene editing in plants aims for absolute control. Microhomology-mediated end joining (MMEJ) offers a promising alternative to existing methods like CRISPR/Cas, potentially advancing crop improvement applications.
Area of Science:
- Plant genetics and molecular biology
- Biotechnology and genetic engineering
Background:
- Achieving absolute precision and control in gene editing and allele introgression is a key goal in genetic engineering.
- Current precision genome editing methods in plants include oligonucleotide-directed mutagenesis (ODM), base editing, prime editing, and homologous recombination (HR)-based gene targeting.
- CRISPR/Cas systems have significantly improved HR-mediated precise editing frequencies by enabling targeted DNA breaks, but further advancements are needed for commercial viability.
Purpose of the Study:
- To summarize recent advancements in precision genome editing mediated by microhomology-mediated end joining (MMEJ).
- To discuss the potential applications of MMEJ-mediated genome editing in crop improvement.
Main Methods:
- Review of recent scientific literature on microhomology-mediated end joining (MMEJ) for plant genome editing.
- Analysis of MMEJ's mechanisms and efficiency compared to other precision editing techniques.
- Exploration of MMEJ's applicability in crop genetic engineering.
Main Results:
- Microhomology-mediated end joining (MMEJ) presents an alternative innovative method for genome editing.
- MMEJ-mediated editing shows potential for precise genetic modifications in plants.
- The review highlights progress and discusses MMEJ's role in future crop improvement strategies.
Conclusions:
- Microhomology-mediated end joining (MMEJ) is an emerging and valuable tool for precision genome editing in plants.
- Further research into MMEJ is crucial for its successful commercial application in crop improvement.
- MMEJ offers a complementary approach to existing gene editing technologies, expanding the toolkit for plant genetic engineering.
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