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Related Experiment Video

Updated: Dec 6, 2025

Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: May 26, 2023

Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal

Tongcheng Qian¹, Daniel A Gil, Emmanuel Contreras Guzman

¹Morgridge Institute for Research, Madison, WI 53715, USA. tqian5@wisc.edu.

|October 13, 2020

Summary

Researchers developed an adaptable pump (Adapt-Pump) to create pulsatile blood flow from cardiac spheroids. This system models endothelial cell (EC) behavior under various shear stresses, revealing abnormal EC organization in disease models.

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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

Area of Science:

Cardiovascular Research
Cell Biology
Biomedical Engineering

Background:

Endothelial cells (ECs) are crucial for cardiovascular health, constantly influenced by mechanical forces like fluidic shear stress from blood flow.
Understanding EC behavior under diverse shear stress conditions requires advanced methods to generate physiologically and pathologically relevant pulsatile flows.

Purpose of the Study:

To introduce and validate an adaptable pump (Adapt-Pump) platform for generating pulsatile flows using human pluripotent stem cell-derived cardiac spheroids (CS).
To investigate endothelial cell responses to varying mechanical microenvironments simulated by the Adapt-Pump system.

Main Methods:

Development of the Adapt-Pump platform capable of generating pulsatile flows from CS.
Utilizing quantitative imaging-based signal transduction to analyze CS contraction characteristics.
Differentiating and exposing ECs to pulsatile flows generated under normal and pathological (long QT syndrome) conditions.

Main Results:

The Adapt-Pump system successfully recapitulated unique CS contraction patterns and drug responses.
Simulations showed CS contraction changes in response to fluidic mechanical stimulation.
ECs exposed to pathological pulsatile flow derived from long QT syndrome models exhibited abnormal monolayer organization.

Conclusions:

The Adapt-Pump platform is a powerful tool for modeling the cardiovascular system and studying EC behavior.
This system enhances our understanding of how mechanical microenvironments affect endothelial cells, with implications for cardiovascular disease research.