Pontibacter fetidus sp. nov. and Pontibacter burrus sp. nov., isolated from the soil

Two novel strains, BT213^T and BT327^T, were isolated from the soil collected in Uijeongbu city, Korea. Cells of strains were Gram negative, aerobic, and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains BT213^T and BT327^T formed two distinct lineages within the family Hymenobacteraceae (order Cytophagales, class Cytophagia). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BT213^T and BT327^T belonged to the genus Pontibacter. Strain BT213^T showed the highest similarities of 97.8% with Pontibacter deserti JC215^T. Strain BT327^T showed the highest sequence similarity of 96.5% with Pontibacter mucosus PB3^T. Optimal growth occurred at 25 °C, pH 7, and in the absence of NaCl. The major cellular fatty acid of strains BT213^T and BT327^T were iso-C_15:0 and summed feature 4 (iso-C_17:1 I/anteiso-C_17:1 B). Strains BT213^T and BT327^T had MK-7 as major respiratory quinone and phosphatidylethanolamine as major polar lipids. The genome size of strains BT213^T and BT327^T were 4,072,018 bp and 4,314,171 bp, respectively. The genomic G + C mol% of strains BT213^T and BT327^T are 45.6% and 46.1%, respectively. Based on biochemical, chemotaxonomic, and phylogenetic analysis, two novel species Pontibacter fetidus BT213^T (KCTC 72345^T = NBRC 114379^T) and Pontibacter burrus BT327^T (KCTC 72412^T = NBRC 114376^T) are proposed as type strains.