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Ultrahigh throughput beehive-like device for blood plasma separation.
Fengtao Jiang1, Nan Xiang1, Zhonghua Ni1
1School of Mechanical Engineering, and Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing, P. R. China.
Electrophoresis
|October 13, 2020
Summary
We developed a low-cost, rapid-prototyping microfluidic device for efficient blood plasma separation. This novel beehive-like design achieves ultrahigh throughput, enabling faster disease diagnosis from large blood volumes.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Biotechnology
Background:
- High-throughput blood plasma separation is crucial for disease diagnosis.
- Existing methods can be time-consuming and costly.
- Microfluidic devices offer potential for rapid and efficient separation.
Purpose of the Study:
- To develop a low-cost, rapid-prototyping, multilayer polymer microfluidic device.
- To achieve ultrahigh-throughput blood plasma separation.
- To optimize device design for efficient particle focusing and plasma separation.
Main Methods:
- Exploration of microchannel dimensions and operational parameters for particle focusing.
- Validation of plasma separation performance using blood samples with varying hematocrits (HCTs).
- Fabrication of a multilayer microfluidic device using laser cutting and thermal lamination.
Main Results:
- Achieved 99% separation efficiency for blood with 0.5% HCT at 1 mL/min throughput.
- Developed a novel beehive-like multiplexing channel arrangement.
- Demonstrated a total processing throughput of up to 72 mL/min with a plasma separation ratio near 90%.
Conclusions:
- The developed microfluidic device enables low-cost, rapid, and ultrahigh-throughput blood plasma separation.
- The device design is suitable for processing large blood volumes for downstream applications.
- This technology holds potential for advancing disease diagnosis.
