Next-generation DNA sequencing for infected genitourinary implants: How I do it
Paul H Chung1, Joon Yau Leong, Seth Teplitsky
1Department of Urology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia Pennsylvania, USA.
The Canadian Journal of Urology
|October 13, 2020
Summary
Infections of genitourinary prostheses can be hard to diagnose with traditional cultures. Next-generation sequencing (NGS) offers a promising new method to identify bacteria in explanted devices, improving treatment accuracy.
Area of Science:
- Urology
- Infectious Diseases
- Genomic Medicine
Background:
- Prosthetic device infections, such as artificial urinary sphincters and inflatable penile prostheses, are severe complications impacting patient quality of life.
- Revision surgeries increase infection risk, yet traditional culture methods for diagnosing these infections are often inconclusive (up to 33% of cases).
Purpose of the Study:
- To describe the institutional experience using next-generation sequencing (NGS) for diagnosing infections in genitourinary prostheses.
- To highlight methods for enhancing the diagnostic yield of NGS in prosthetic device infections.
Main Methods:
- Utilized next-generation sequencing (NGS) to analyze microbial DNA from explanted genitourinary prosthetic devices.
- Developed and refined techniques to maximize the identification of bacterial composition in infected devices.
Main Results:
- NGS demonstrated potential in identifying microbial culprits in prosthetic device infections where traditional cultures were inconclusive.
- The study outlines specific institutional practices that improve the utility and diagnostic accuracy of NGS.
Conclusions:
- Next-generation sequencing (NGS) presents a valuable advancement for diagnosing prosthetic device infections, overcoming limitations of traditional cultures.
- Implementing optimized NGS techniques can significantly enhance the identification of pathogens, guiding more effective antimicrobial therapy for genitourinary prosthesis infections.
