Ultrasonic vocalizations and individual differences in rats performing a Pavlovian conditioned approach task

Rats emit distinct types of ultrasonic vocalizations (USV), including high-frequency 50-kHz USV, which occur in appetitive situations. Such 50-kHz USV are thought to reflect positive affective states, for example in case of reward anticipation, and are linked to dopamine signaling. The present study was conducted to investigate whether rats emit 50-kHz USV during a Pavlovian conditioned approach task and whether trait-like differences in 50-kHz USV emission are associated with sign- versus goal-tracking. We hypothesize that individuals engaging more with a cue predicting a food reward will also elicit more 50-kHz USV. In order to test this, we investigated 34 female rats and gauged USV while they underwent a Pavlovian conditioned approach training and extinction paradigm. For one, we found a high subject-dependent variability in the emission of 50-kHz calls. These were not largely affected by state differences, since these 50-kHz USV were observed throughout the task. During task progress and in most subjects, there was a rather complete shift toward goal-tracking, but subjects engaging more with the cue predicting a reward also emitted higher numbers of appetitive 50-kHz calls. This supports the hypothesis that sign-tracking is positively associated with the emission of 50-kHz USV. The high subject-dependent variability in the emission of 50-kHz calls warrants special attention in future appetitive studies.