Antagonistic Impact of Acrylamide and Ethanol on Biochemical and Morphological Parameters Consistent with Bone Health
Monika Martiniakova1, Anna Sarocka1, Veronika Kovacova1
1Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, 949 74 Nitra, Slovakia.
Abstract:
The aim of present study was to verify antagonistic effect of acrylamide (AA) and ethanol (Et) on bone quality parameters. Adult mice (n = 20) were segregated into four groups following 2 weeks administration of toxins: group E1, which received AA (20 mg/kg body weight daily); group E2, which received 15% Et (1.7 g 100% Et/kg body weight daily); group E12, which received simultaneously both toxins; and a control group. An insignificant impact of individual applications of AA, Et or their simultaneous supplementation on the total body weight of mice and the length and weight of their femoral bones was identified. In group E1, higher levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), a decreased level of glutathione (GSH) and elevated endocortical bone remodelling were determined. A significantly lower relative volume of cortical bone, bone mineral density (BMD), elevated endocortical bone remodelling and cortical porosity, higher levels of ALT, AST, lower values for total proteins (TP), GSH, alkaline phosphatase (ALP), calcium, and phosphorus were recorded in group E2. In the mice from group E12, the highest endocortical bone remodelling, decreased values for BMD, TP, GSH and ALP and increased levels of ALT and AST were found. Our findings confirmed the antagonistic impact of AA and Et at doses used in this study on biochemical and morphological parameters consistent with bone health in an animal model.
Insights
Acrylamide and ethanol negatively impact bone health, affecting bone mineral density and remodeling. Simultaneous exposure to both toxins exacerbated these adverse effects in mice.
Area of Science:
- Toxicology
- Biochemistry
- Orthopedics
Background:
- Acrylamide (AA) and ethanol (Et) are common environmental toxins.
- Both AA and Et have been individually linked to adverse health effects.
- The combined effect of AA and Et on bone quality is not well understood.
Purpose of the Study:
- To investigate the antagonistic effects of acrylamide and ethanol on bone quality parameters in an adult mouse model.
- To assess the impact of individual and combined exposure to AA and Et on biochemical markers and bone morphology.
Main Methods:
- Adult mice (n=20) were divided into four groups: control, AA-only, Et-only, and combined AA+Et.
- Toxins were administered daily for two weeks.
- Bone quality parameters, including bone mineral density (BMD), cortical bone volume, porosity, and biochemical markers (ALT, AST, TG, GSH, TP, ALP, calcium, phosphorus), were analyzed.
Main Results:
- Individual AA or Et exposure and combined exposure did not significantly affect body weight or femoral bone length/weight.
- AA exposure increased ALT, AST, TG, and endocortical bone remodeling, while decreasing GSH.
- Et exposure significantly reduced cortical bone volume, BMD, and increased endocortical bone remodeling and porosity, alongside altered biochemical markers.
- Combined AA+Et exposure showed the most pronounced negative effects, with highest bone remodeling and decreased BMD, TP, GSH, and ALP.
Conclusions:
- Acrylamide and ethanol exhibit antagonistic effects on bone quality parameters in mice.
- Combined exposure to AA and Et exacerbates negative impacts on bone health compared to individual exposures.
- The findings highlight the potential risks of co-exposure to these toxins for bone health.
