Roughage to Concentrate Ratio and Saccharomyces cerevisiae Inclusion Could Modulate Feed Digestion and In Vitro...

The objective of this research was to investigate the effect of the roughage-to-concentrate (R:C) ratio and the addition of live yeast (LY) on ruminal fermentation characteristics and methane (CH₄) production. The experimental design was randomly allocated according to a completely randomized design in a 4 × 4 factorial arrangement. The first factor was four rations of R:C at 80:20, 60:40, 40:60, and 20:80, and the second factor was an additional four doses of Saccharomyces cerevisiae (live yeast; LY) at 0, 2.0 × 10⁶, 4.0 × 10⁶, and 6.0 × 10⁶ colony-forming unit (cfu), respectively. For the in vitro method, during the incubation, the gas production was noted at 0, 1, 2, 4, 6, 8, 10, 12, 18, 24, 48, 72, and 96 h. The rumen solution mixture was collected at 0, 4, 8, 12, and 24 h of incubating after inoculation. Cumulative gas production at 96 h was highest in the R:C ratio, at 20:80, while the addition of LY improves the kinetics and accumulation of gas (p > 0.05). Maximum in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD) at 24 h after incubation were achieved at the R:C ratio 20:80 and the addition of LY at 6 × 10⁶ cfu, which were greater than the control by 13.7% and 12.4%, respectively. Ruminal pH at 8 h after incubation decreased with an increased proportion of concentrates in the diet, whereas it was lowest when the R:C ratio was at 20:80. Increasing the proportion of a concentrate diet increased total volatile fatty acid (TVFA) and propionic acid (C3), whereas the acetic acid (C2) and C2-to-C3 ratios decreased (p < 0.05). TVFA and C3 increased with the addition of LY at 6 × 10⁶ cfu, which was greater than the control by 11.5% and 17.2%, respectively. No interaction effect was observed between the R:C ratio and LY on the CH₄ concentration. The calculated ruminal CH₄ production decreased with the increasing proportion of concentrates in the diet, particularly the R:C ratio at 20:80. The CH₄ production for LY addition at 6 × 10⁶ cfu was lower than the control treatment by 17.2%. Moreover, the greatest populations of bacteria, protozoa, and fungi at 8 h after incubation were found with the addition of LY at 6 × 10⁶ cfu, which were higher than the control by 19.0%, 20.7%, and 40.4%, respectively. In conclusion, a high ratio of roughage and the concentrate and addition of LY at 6.0 × 10⁶ cfu of the total dietary substrate could improve rumen fermentation, improve feed digestibility, and reduce the CH₄ production.