Related Experiment Video
Updated: Dec 6, 2025

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
Published on: October 20, 2014
Cell Signaling Pathway Reporters in Adult Hematopoietic Stem Cells
Jolanda J D de Roo1, Frank J T Staal1
1Department of Immunology, L3-Q, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands.
Abstract:
Hematopoietic stem cells (HSCs) develop at several anatomical locations and are thought to undergo different niche regulatory cues originating from highly conserved cell signaling pathways, such as Wnt, Notch, TGF-β family, and Hedgehog signaling. Most insight into these pathways has been obtained by reporter models and loss- or gain of function experiments, yet results differ in many cases according to the approach. In this review, we discuss existing murine reporter models regarding these pathways, considering the genetic constructs and reporter proteins in the context of HSC studies; yet these models are relevant for all other stem cell systems. Lastly, we describe a multi-reporter model to properly study and understand the cross-pathway interaction and how reporter models are highly valuable tools to understand complex signaling dynamics in stem cells.
More Related Videos
12:44Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Published on: November 11, 2014
12:03Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Published on: July 8, 2012
Related Concept Videos
Regulation of Hematopoietic Stem Cells
Lineage Commitment
Multipotency of Hematopoietic Stem Cells
Adult Stem Cells
Hematopoiesis
Stem Cell Niche