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Updated: Dec 6, 2025

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture
Published on: October 23, 2019
Transcriptome sequencing of cochleae from constant-frequency and frequency-modulated echolocating bats
Lu Ma1,2, Haijian Sun1, Xiuguang Mao3,4
1School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200062, China.
Researchers sequenced bat cochlear transcriptomes to understand ultrahigh frequency hearing. This study provides a foundational dataset for future research into bat echolocation and hearing mechanisms.
Area of Science:
- * Bioacoustics and Sensory Biology
- * Genomics and Transcriptomics
Background:
- * Echolocating bats utilize ultrahigh frequency hearing for navigation and hunting in darkness.
- * Understanding the molecular basis of bat hearing is crucial for deciphering their sensory capabilities.
Purpose of the Study:
- * To generate full-length (FL) transcriptomes from the cochlear tissues of echolocating bats.
- * To create a comprehensive dataset of transcripts associated with ultrahigh frequency hearing.
Main Methods:
- * PacBio single-molecule real-time isoform sequencing (Iso-seq) technology was employed.
- * Cochlear tissues from constant-frequency (CF) bats (Rhinolophus affinis subspecies) and frequency-modulated (FM) bats (Myotis ricketti) were analyzed.
Main Results:
- * Generated 10103, 9676, and 10504 non-redundant FL transcripts for R. a. hainanus, R. a. himalayanus, and Myotis ricketti, respectively.
- * A total of 26342 FL transcripts were identified, with 24833 annotated in public databases.
- * The dataset includes transcripts crucial for ultrahigh frequency hearing in echolocating bats.
Conclusions:
- * This study provides a valuable, reusable dataset for studying bat hearing and echolocation.
- * The generated transcriptomes can be used for gene expression analysis, alternative splicing assessment, novel transcript identification, and genome annotation improvement.
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