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Updated: Dec 6, 2025

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

Protein-crystal detection with a compact multimodal multiphoton microscope.

Qing-di Cheng¹, Hsiang-Yu Chung^2,3, Robin Schubert^1,4

¹Laboratory for Structural Biology of Infection and Inflammation, University of Hamburg, c/o DESY, Building 22a Notkestrasse 85, 22607, Hamburg, Germany.

Communications Biology

|October 14, 2020

Summary

This summary is machine-generated.

A new multimodal microscope rapidly detects protein crystals using nonlinear optical imaging. This technology enhances sample screening for serial diffraction, boosting biomolecular crystallography productivity.

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Last Updated: Dec 6, 2025

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

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Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions

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Published on: January 30, 2018

Area of Science:

Biomolecular crystallography
Microscopy
Photonics

Background:

Increasing demand for rapid protein crystal detection methods for serial diffraction.
Limitations of current methods in sensitivity and speed.

Purpose of the Study:

To demonstrate a compact multimodal microscope for sensitive protein crystal identification.
To support sample scoring and optimization in biomolecular crystallography.

Main Methods:

Utilized a fiber-based ultrafast laser for excitation at 775 nm and 1300 nm.
Employed nonlinear optical imaging techniques: second-harmonic generation, third-harmonic generation, and three-photon excited ultraviolet fluorescence.
Developed a compact, multimodal, multiphoton microscope.

Main Results:

Successfully identified and detected protein micro- and nano-crystal suspensions with high sensitivity.
Simultaneous recording of multiple nonlinear optical signals.
Demonstrated the instrument's capability for effective sample screening.

Conclusions:

The developed microscope is a valuable tool for biomolecular crystallography.
It enhances capabilities and productivity in serial diffraction data collection.
This technology promises to advance protein crystal analysis.