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Steric hindrance: A practical (and frequently forgotten) problem in flow cytometry

Daniel Mazza Matos1

  • 1Flow Cytometry Section; Cell Processing Center (CPC), Center of Hematology and Hemotherapy of Ceará (HEMOCE), Fortaleza, Ceará, Brazil.

Cytometry. Part B, Clinical Cytometry
|October 14, 2020
PubMed
Summary

No abstract available in PubMed .

Keywords:
flow cytometryimmunophenotypingquenchingsteric hindrancesurface immunoglobulin light-chain

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Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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