Rheinheimera mangrovi sp. nov., a bacterium isolated from mangrove sediment

A Gram-stain-negative, rod-shaped, motile and strictly aerobic bacterium, designated LHK132^T, was isolated from a mangrove sediment sample collected in Haikou, Hainan Province, PR China. Strain LHK132^T was able to grow at temperatures of 10-45 °C, at salinities of 0-7.0 % (w/v) and at pH 6.0-9.0. Catalase and oxidase activities, H₂S production, urease and methyl red reaction were positive. Indole, nitrate reduction, hydrolysis of gelatin, starch, casein and Tweens 20, 40, 60 and 80 were negative. The major cellular fatty acids were C_16 : 0 and summed feature 3 (C_16 : 1  ω7c and/or C_16 : 1  ω6c). The only respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. According to 16S rRNA gene sequence analysis, strain LHK132^T had 98.3, 97.5, 97.4, 97.2 and 97.1% similarities to Rheinheimera soli BD-d46^T, Rheinheimera sediminis YQF-1^T, Rheinheimera tangshanensis JA3-B52^T, Rheinheimera mesophila IITR-13^T and Rheinheimera arenilitoris J-MS1^T, respectively. Phylogenetic analyses indicated that strain LHK132^T formed a distinct lineage with R. soli BD-d46^T within the genus Rheinheimera. The average nucleotide identity and digital DNA-DNA hybridization values between strain LHK132^T and related species of the genus Rheinheimera were well below the thresholds for species delineation. The DNA G+C content was 46.7 mol%. On the basis of its phenotypic, chemotaxonomic and genotypic data, strain LHK132^T is considered a representative of a novel species in the genus Rheinheimera, for which the name Rheinheimera mangrovi sp. nov. is proposed. The type strain is LHK132^T (=KCTC 62580^T=MCCC 1K03529^T).