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Updated: Dec 5, 2025

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
Published on: March 2, 2014
BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation
Nicolas Maldonado1, Fernanda Fredericksen2, Constanza Espiñeira2
1Facultad De Ciencias, Instituto De Bioquímica y Microbiología, Universidad Austral De Chile, Campus Isla Teja S/N, Valdivia, Chile; Departamento De Ciencias Biológicas y Química. Facultad De Medicina y Ciencia, Universidad San Sebastián, Lago Panguipulli 1390, Puerto Montt, Chile.
Abstract:
The bovine viral diarrhea virus (BVDV-1) is a pathogen with the capacity to modulate the interferon type I system. To further investigate the effects of BVDV-1 on the production of the immune response, the Madin-Darby bovine kidney cell line was infected with the cytopathic CH001 field isolate of BVDV-1, and the IFNbeta expression profiles were analyzed. The results showed that cpBVDV-1 was able to induce the production of IFNbeta in a way similar to polyinosinic-polycytidylic acid, but with less intensity. Interestingly, all cpBVDV-1 activities were blocked by pharmacological inhibitors of the IRF-1, IRF-7, and NF-κB signaling pathway, and the level of IFNbeta decreased at the level of transcript and protein. These results, together with in silico analyses showing the presence of several regulatory consensus target motifs, suggest that cpBVDV-1 regulates IFNbeta expression in bovines through the activation of several key transcription factors. Collectively, the results suggest that during cpBVDV-1 infection, cross talk is evident between various signaling pathways involved in transcriptional activation of IFNbeta in cattle.
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