Precise Genome Editing in Poultry and Its Application to Industries

Area of Science:

• Genetics and Genomics
• Animal Science
• Biotechnology

Background:

• Poultry, particularly chickens, are vital model organisms in food production, developmental biology, and biomedicine.
• Genome editing offers a powerful tool to engineer novel traits in poultry.
• CRISPR/Cas9 technology has rapidly advanced genome editing capabilities.

Purpose of the Study:

• To summarize recent advancements in CRISPR/Cas9 genome editing for avian species.
• To highlight the application of these techniques in creating genome-edited poultry models.
• To discuss the potential industrial applications of genome-edited poultry.

Main Methods:

• Utilizing CRISPR/Cas9 gene editing systems.
• Employing cultured primordial germ cells (PGCs) for germline transmission.
• Leveraging viral vector systems for efficient delivery of editing components.

Main Results:

• Successful generation of genome-edited poultry models with specific novel traits.
• Demonstration of CRISPR/Cas9 efficacy in avian species.
• Establishment of methods combining genome editing with PGCs and viral vectors.

Conclusions:

• CRISPR/Cas9 technology is revolutionizing the creation of poultry models.
• Genome-edited poultry hold significant promise for basic sciences, agriculture, and the biomedical industry.
• Further applications of genome-edited poultry across various sectors are anticipated.