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Updated: Dec 5, 2025

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
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Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Published on: October 2, 2017

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Crystal Structure of Mannose Specific IIA Subunit of Phosphotransferase System from Streptococcus pneumoniae

Malgorzata Magoch1,2, Przemyslaw Nogly3,4, Przemyslaw Grudnik1

  • 1Malopolska Centre of Biotechnology, Jagiellonian University, 30-387 Krakow, Poland.

Molecules (Basel, Switzerland)
|October 15, 2020
PubMed

Insights

Researchers determined the structure of a key protein in Streptococcus pneumoniae, the mannose phosphotransferase system (Man-PTS). This finding aids in developing new antibiotics against this common bacterial pathogen.

Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • Streptococcus pneumoniae causes severe respiratory tract infections, posing a significant global health challenge.
  • This bacterium relies on carbohydrate fermentation for energy and utilizes the mannose phosphotransferase system (Man-PTS) for hexose transport and virulence gene regulation.

Purpose of the Study:

  • To determine the three-dimensional structure of the EIIA domain of the Streptococcus pneumoniae mannose phosphotransferase system (SpEIIA-Man).
  • To provide a detailed molecular description of the SpEIIA-Man active site.

Main Methods:

  • X-ray crystallography was used to determine the three-dimensional structure of SpEIIA-Man.
  • Structural analysis focused on the dimeric arrangement and active site of the protein.

Main Results:

  • The three-dimensional structure of SpEIIA-Man was elucidated, revealing a dimeric arrangement.
  • A detailed molecular description of the SpEIIA-Man active site was provided.

Conclusions:

  • The structural data of SpEIIA-Man provides a foundation for developing novel antimicrobial strategies.
  • Targeting PTS transporters, which are microbial-specific, presents a promising avenue for antistreptococcal antibiotic development.
Keywords:
IIA subunitStreptococcus pneumoniaeX-ray crystallographymannose PTSphosphotransferase systemsugar transport

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