Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Positive Regulator Molecules02:39

Positive Regulator Molecules

6.3K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
6.3K
Positive Regulator Molecules01:45

Positive Regulator Molecules

126.4K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
126.4K
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

6.1K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
6.1K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

5.4K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.4K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

7.5K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.5K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

4.1K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
4.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AURORA A interacts with DICER and SETD2 to promote S-phase progression.

EMBO reports·2026
Same author

A murine cytomegalovirus cell cycle regulator (m54.5p) evolved within the conserved viral DNA polymerase gene.

PLoS pathogens·2026
Same author

Tubulin polyglutamylases TTLL4C and TTLL6B are essential for maintaining cytoskeletal integrity in <i>Trypanosoma brucei</i>.

mSphere·2026
Same author

Structure Merging Approach Leads to New Dual Potent and Selective USP25/USP28 Inhibitors.

Journal of medicinal chemistry·2026
Same author

Immunoglobulin G complexes from post-infectious ME/CFS, including post-COVID ME/CFS disrupt cellular energetics and alter inflammatory marker secretion.

Brain, behavior, & immunity - health·2026
Same author

Cooperative clamp-mediated promoter recognition by poxviral RNA polymerase and its TBP/TFIIB-like partner.

Nature communications·2026

Related Experiment Video

Updated: Dec 5, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.1K

Structural basis for CDK7 activation by MAT1 and Cyclin H.

Stefan Peissert1, Andreas Schlosser1, Rafaela Kendel1

  • 1Rudolf Virchow Center for Integrative and Translational Bioimaging, Institute for Structural Biology, University of Würzburg, 97080 Würzburg, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|October 15, 2020
PubMed
Summary

The CDK-activating kinase (CAK) complex structure reveals how MAT1 binding activates CDK7, independent of T-loop phosphorylation. This finding clarifies CAK complex regulation for cell-cycle control and transcription.

Keywords:
CDK-activating kinasecell-cycle controlkinasetranscription

More Related Videos

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.7K
Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

12.2K

Related Experiment Videos

Last Updated: Dec 5, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.1K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.7K
Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

12.2K

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • The CDK-activating kinase (CAK) complex, comprising CDK7, Cyclin H, and MAT1, is crucial for transcription and cell-cycle regulation.
  • CAK activates RNA polymerase II via TFIIH and phosphorylates CDK T-loops, highlighting its dual regulatory roles.

Purpose of the Study:

  • To elucidate the structural basis of CAK complex assembly and activation.
  • To understand the mechanism of CDK7 activation by its partners, MAT1 and Cyclin H.

Main Methods:

  • X-ray crystallography to determine the CAK complex structure from *Chaetomium thermophilum* at 2.6-Å resolution.
  • In vitro activity assays and functional mutagenesis to investigate CDK7 activation mechanisms.

Main Results:

  • The structure reveals detailed interactions between CDK7, MAT1, and Cyclin H, explaining CAK complex regulation.
  • CDK7 activation is shown to be dependent on MAT1, which positions the CDK7 T-loop in an active conformation, independent of T-loop phosphorylation.

Conclusions:

  • The study provides a structural foundation for understanding CDK7 activation and CAK complex regulation.
  • MAT1 plays a key role in activating CDK7, independent of T-loop phosphorylation, offering insights into cell-cycle and transcription control.