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Related Experiment Video

Updated: Dec 5, 2025

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Published on: October 11, 2012

Programmed Cell Death 2-Like (Pdcd2l) Is Required for Mouse Embryonic Development.

Brendan J Houston¹, Manon S Oud², Daniel M Aguirre³

¹School of Biological Sciences, Monash University, Clayton, Australia brendan.houston@monash.edu.

G3 (Bethesda, Md.)

|October 15, 2020

Summary

This summary is machine-generated.

Programmed cell death protein 2 like (PDCD2L) is crucial for embryonic development, not male fertility. Knockout mice and fruit flies lacking PDCD2L exhibit developmental lethality, indicating its conserved role in development.

Keywords:

Pdcd2l acrosome embryonic development male infertility sperm function

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Area of Science:

Developmental Biology
Genetics
Reproductive Biology

Background:

Globozoospermia, a rare infertility cause, is linked to globozoospermia gene mutations.
Previous whole exome screening identified homozygous mutations in PDCD2L as a potential cause of globozoospermia.

Purpose of the Study:

To investigate the role of PDCD2L in male fertility using a knockout mouse model.
To determine the function of PDCD2L in germ cells using Drosophila melanogaster.

Main Methods:

Generated Pdcd2l knockout mice to assess male fertility.
Utilized Drosophila melanogaster to study the role of the PDCD2L ortholog (trus) in germ cells via knockdown experiments.

Main Results:

Pdcd2l knockout mice exhibited embryonic lethality, with embryos resorbed between embryonic days 12.5-17.5.
Heterozygous Pdcd2l males showed normal fertility, comparable to wildtype males.
Germ cell-specific knockdown of trus in Drosophila did not impact male fertility, despite global knockdown causing larval lethality.

Conclusions:

PDCD2L is essential for embryonic development, not male fertility.
The data suggest an evolutionarily conserved role for PDCD2L in development across species.