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A dumbbell probe-based dual signal amplification strategy for sensitive detection of multiple DNA methyltransferases
Yueying Li1, Shuli Sun, Xiaorui Tian
1College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. cyzhang@sdnu.edu.cn.
Abstract:
We demonstrate for the first time the integration of a dumbbell probe with dual signal amplification for the simultaneous detection of multiple DNA methyltransferases (MTases). This method is very sensitive with a detection limit of 2.15 × 10-5 U mL-1 for DNA adenine methyltransferase (Dam) and 3.23 × 10-6 U mL-1 for CpG Methyltransferase (M.SssI), and it can be used to screen the enzyme inhibitors and simultaneously measure Dam and M.SssI in complex biological samples.
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