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Conditional surrender in one generation: determining the reproductive roles of mouse embryo lethal genes by embryo
1Dept. of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA.
Biology of Reproduction
|October 15, 2020
Summary
The laboratory mouse remains a premier model for mammalian development research. Advanced genomic tools, including CRISPR/Cas9, enhance its utility for studying complex biological processes and human diseases.
Area of Science:
- * Developmental Biology
- * Genetics
- * Mammalian Reproduction
Background:
- * The laboratory mouse has been the dominant animal model for mammalian development and reproduction studies for decades.
- * Embryonic stem cell (ESC) gene targeting was a key factor in establishing the mouse model.
- * The development of sophisticated genomic manipulation techniques further solidified the mouse's role.
Purpose of the Study:
- * To highlight the enduring importance of the mouse as a model organism.
- * To discuss the impact of new genome editing technologies on model systems.
- * To emphasize the mouse's continued relevance for studying mammalian development and disease.
Main Methods:
- * Review of historical and current methodologies in mouse genetics.
- * Analysis of the impact of embryonic stem cell technology.
- * Evaluation of the influence of CRISPR/Cas9 genome editing.
Main Results:
- * The mouse's pre-eminence was significantly driven by ESC gene targeting capabilities.
- * CRISPR/Cas9 technology has broadened genome editing accessibility across species.
- * Despite new technologies, the mouse retains its status due to established infrastructure.
Conclusions:
- * The laboratory mouse remains the preferred model for investigating mammalian development and reproduction.
- * Established infrastructure and historical data make it invaluable for molecular mechanism studies.
- * It is crucial for modeling human development and associated diseases.
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