Optimal fermentation time for Nigrospora-fermented tea rich in bostrycin

Area of Science:

• Food Science
• Microbiology
• Biotechnology

Background:

• Bostrycin, known for anticancer properties, is produced by Nigrospora sphaerica HCH285.
• Nigrospora-fermented tea is developed using Camellia sinensis green tea and acclimated HCH285 strain.

Purpose of the Study:

• Investigate the impact of fermentation duration on bostrycin production by Nigrospora sphaerica HCH285.
• Determine the optimal fermentation period for developing a high-quality Nigrospora-fermented tea.

Main Methods:

• Fermentation of sun-dried green tea using Nigrospora sphaerica HCH285.
• Analysis of bostrycin content, sensory qualities, and chemical composition over time.
• Acute oral toxicity testing of the fermented tea.

Main Results:

• Maximum bostrycin content (3.18 g/kg) achieved at 45 days of fermentation.
• Optimal sensory quality (color, aroma, taste) observed at 50 days.
• Decreased levels of amino acids, polyphenols, and sugars; increased water-soluble substances; non-toxic profile.

Conclusions:

• Optimal fermentation time for Nigrospora-fermented tea is 45-50 days.
• Findings support the development of tea biotechnology and novel functional tea products.
• Nigrospora-fermented tea is a safe and potentially valuable new product.