Comparative evaluation of some enzymic digestion procedures in the release of basic drugs from tissue

The relative efficiency of four enzymic-digestion procedures in the release of eight basic drugs from tissue has been studied. Enzymes employed include: subtilisin Carlsberg, trypsin, papain, and neutrase. The results obtained on recovery show that papain digestion gives highest recovery for most of the drugs studied. Papain is suggested as the enzyme of choice. The application of neutrase, a neutral proteinase from B. subtilis, is reported for the first time.