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2-Methacryloyloxyethyl Phosphorylcholine Polymer Treatment of Complete Dentures to Inhibit Denture Plaque Deposition
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Inhibitory effects of 2-methacryloyloxyethyl phosphorylcholine polymer on the adherence of bacteria causing upper

Hiroyuki Iuchi1, Junichiro Ohori1, Takayuki Kyutoku1

  • 1Department of Otolaryngology, Head and Neck Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.

Journal of Oral Microbiology
|October 16, 2020
PubMed
Abstract

Insights

2-methacryloyloxyethyl phosphorylcholine (MPC) polymer effectively inhibits Streptococcus pneumoniae and nontypeable Haemophilus influenzae adherence, suggesting potential for treating upper respiratory tract infections.

Area of Science:

  • Biomaterials Science
  • Microbiology
  • Infectious Diseases

Background:

  • Upper respiratory tract infections (URTIs) are frequently caused by Streptococcus pneumoniae (Spn) and nontypeable Haemophilus influenzae (NTHi).
  • Bacterial adherence to host tissues is a critical step in the pathogenesis of URTIs.
  • Developing strategies to prevent bacterial adherence is crucial for managing and preventing infections.

Purpose of the Study:

  • To investigate the inhibitory effect of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on the adherence of Spn and NTHi.
  • To evaluate the efficacy of MPC polymer both in vitro and in vivo.
  • To compare the inhibitory effects of MPC polymer with PC-keyhole limpet hemocyanin (PC-KLH).

Main Methods:

  • Evaluated phosphorylcholine (PC) expression of 21 Spn and 21 NTHi strains using fluorescence-activated cell sorting.
  • Determined bacterial adherence to Detroit 562 cells (in vitro) and mouse nasal mucosa (in vivo).
  • Compared MPC polymer-mediated inhibition with PC-KLH-mediated inhibition.

Main Results:

  • MPC polymer significantly inhibited Spn and NTHi adherence in a dose-dependent manner, independent of PC expression.
  • No correlation was found between PC expression and MPC polymer's inhibitory effect.
  • PC-KLH showed a negative correlation between inhibitory effects and PC expression; MPC polymer did not affect nasal mucosa histology.

Conclusions:

  • MPC polymer demonstrates significant potential in reducing Spn and NTHi adherence.
  • MPC polymer could be a valuable component in developing novel local treatments for URTIs.
  • Potential applications include topical gargles and nebulizer medications to prevent infection.
Keywords:
2-Methacryloyloxyethyl phosphorylcholinebalb/c micedetroit 562 cellsinhibitory effectnontypeable Haemophilus influenzaphosphorylcholinestreptococcuspneumonia

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