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Updated: Dec 5, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Research on the circular RNA bioinformatics in patients with acute myocardial infarction
Lianli Yin1, Yinghua Tang2, Minghe Jiang3
1Department of Clinical Laboratory, Nanning Second People's Hospital, the Third Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
Insights
Circular RNAs (circRNAs) and microRNAs interact in acute myocardial infarction (AMI) development. These circRNAs may play a role in the pathogenesis of AMI, offering potential diagnostic and therapeutic targets.
Area of Science:
- Molecular Biology
- Genetics
- Cardiovascular Research
Background:
- Acute myocardial infarction (AMI) is a leading cause of mortality worldwide.
- The underlying molecular mechanisms of AMI are complex and not fully understood.
- Circular RNAs (circRNAs) are emerging as key regulators in various biological processes.
Purpose of the Study:
- To identify circRNAs associated with acute myocardial infarction (AMI).
- To explore the relationship between circRNAs and AMI.
- To investigate the potential mechanisms involving circRNAs in AMI pathogenesis.
Main Methods:
- Expression profiling of circRNAs using microarray gene chips in 3 AMI patients and 3 healthy controls.
- Identification of differentially expressed circRNAs between AMI patients and controls.
- Construction of a circRNA-microRNA (miRNA) regulatory network.
Main Results:
- 650 differentially expressed circRNAs were identified in AMI patients (535 up-regulated, 115 down-regulated).
- Key circRNAs (e.g., hsa_circ_0066439, hsa_circ_0081241, hsa_circ_0122984) were found to regulate signaling pathways via miRNAs (e.g., hsa-miR-1254, hsa-miR-328-5p).
- Differentially expressed circRNAs are implicated in pathways such as MAPK signaling and focal adhesion, relevant to cardiovascular diseases.
Conclusions:
- CircRNA and miRNA interactions are involved in the development of AMI.
- CircRNAs may play a significant role in the pathogenesis of AMI.
- CircRNAs represent potential biomarkers and therapeutic targets for AMI.
Objective:
Through the detection of circular RNA (circRNA) using expression profiling chips, we searched for circRNAs related to acute myocardial infarction (AMI) and explored their relationship and possible mechanisms with AMI.
Method:
The study subjects included 3 AMI patients and 3 controls, and circRNA expression profiling analysis was performed using a microarray gene chip to identify circRNAs with large differences in expression between groups and to construct a circRNA-miRNA network.
Results:
Compared with the control group, there were 650 differentially expressed circRNAs found in AMI patients (P < .05, fold change > 2), including 535 up-regulated circRNAs, such as hsa_circ_0050908, hsa_circRNA4010-22, hsa_circ_0081241, hsa_circ_0010551, hsa_circRNA4010-20, hsa_circRNA14702, hsa_circ_0115392, has_circRNA1825-44, has_circRNA8493-7, and hsa_circ_0025097. Furthermore, there were 115 down-regulated circRNAs, such as hsa_circ_0066439, hsa_circ_0054211, hsa_circ_0095920, hsa_circ_0122984, hsa_circ_0113067, hsa_circ_0039155, hsa_circRNA4014-45, hsa_circ_0122979, hsa_circ_0059665, and hsa_circ_0009319. The circRNAs hsa_circ_0066439, hsa_circ_0081241, and hsa_circ_0122984 can regulate multiple signal pathways to participate in the AMI process through hsa-miR-1254, hsa-miR-328-5p, and other miRNAs. In addition, the expression of circRNA-miRNA in peripheral blood is related to the network. Differentially expressed circRNAs are involved in chromatin organization, chromatin-modifying enzymes, signal transduction, lysine degradation, the mitogen-activated protein kinase (MAPK) signaling pathway, focal adhesion, and a variety of other pathways, such as myocardial infarction, coronary heart disease, hypertension, and other diseases. The gene ontology analysis results show that molecular function mainly involves binding and molecular structural activity, whereas the biological process mainly involves a single biological process, a cellular component for organization, and a cellular process, and the cellular component mainly involves a protein complex, an extracellular matrix, and a membrane.
Conclusion:
circRNA and microRNA interact to participate in the development of AMI. circRNA may be involved in the pathogenesis of AMI.
